The impact of dynamic flow and probiotics on intestinal cell lines

Abstract: The gut microbiome has been well documented as a complex consortium of microbes that are integral to host health and disease, with a healthy gut microbiome shown to protect its host against infection, whereas dysbiosis has been linked to the development of numerous diseases, gastrointestinal and otherwise (Bull and Plummer 2014). However, realistic human gut models are lacking, since standard methods utilise static methodologies such as multi-well culture plates and tissue culture flasks, microbiome-host interaction studies lack physiological conditions seen in situ. In addition, with the use of probiotics, the focus on the gut as a site of complex host-microbe interactions is highlighted, with probiotics acting as another means of improving general gut health and aiding the microbiome in times of dysbiosis and disease (Shimizu et al. 2013; Wischmeyer et al. 2016).

Utilising the Kirkstall Quasi Vivo® dynamic flow through system to emulate the natural flow found in situ, in tandem with a probiotic mix (Lab4 and Lab4B), the key aim would be to provide an improved gut model, in which a host-microbe interaction would be emulated in the presence of flow. By measuring the impact to both cell viability and mucin production, with four human intestinal cell lines, it would reveal the impact that these elements provide to the human gut, be that in improving cellular parameters, or worsening them. Furthermore, by emulating distinct factors seen in situ, this research could reveal any discrepancies between standard tissue culture modelling and this novel methodology, thus presenting an improved model of the human gut for use in future research.