EpiIntestinal on a Chip: Label-free Microphysiometry
Microphysiometry showed to be a useful tool to monitor the energy metabolism of living cells and its interaction with living cells. In the past the technique was mainly used for monitoring of 2D monolayers of living cells . Recently, our group showed that it is also possible to monitor the extracellular acidification rate (EAR) and transepithelial electrical resistance (TEER) of 3D skin constructs in an automated assay maintaining an air liquid interface (ALI) with the IMOLA-IVD technology .
In this work we present an Intestine-on-a-Chip by monitoring EAR and TEER of the MatTek 3D-small intestinal tissue model (EpiIntestinal) for 12 h. A periodic cycle of 97 min ALI, 8 min TEER measurement and 15 min washing step was used. The test substance (0.2 % sodium dodecyl sulfate) was applied after 8 h of measurement. After the application of the test substance a reduction of the EAR and a change in TEER could be monitored. To be able to monitor the EAR a low buffered basal medium was used. The presented work shows a proof of principle of automated monitoring of EAR and TEER at a 3D intestine model maintaining an automated ALI. The EpiIntestinal model on the IMOLA-IVD chip is a promising research tool for the use in the field of toxicology, cellular metabolism studies or drug absorption research.